My research focuses on the study of biomolecular interactions using
Isothermal Titration Calorimetry (ITC). ITC directly measures the heat
associated with most biomolecular interactions to determine
thermodynamic parameters including binding affinity, stoichiometry, and
binding energetics to lead us to a better understanding of molecular
interactions. Systems studied so far include the interactions of
proteins, peptides, antibiotics, amino acids, drugs and drug candidates
with small molecules and metal ions. Such studies also contribute to
better drug designs and testing.
Selected Publications
Jin, L., Sakiyan, I., Gonzales, N. S., Lane, D., and Cherala, S. S. “Synthesis, Characterization and Cu
2+ Binding Studies of L-Histidine Ester of 8-Hydroxyquinoline”
Inorg. Chim. Acta, (2014)
linkStrawn,
Rebecca; Stockner, Thomas; Melichercik, Milan; Jin, Lihua; Xue,
Wei-Feng; Carey, Jannette; Ettrich, Rudiger; "Synergy of molecular
dynamics and isothermal titration calorimetry in studies of allostery;"
Methods in Enzymology (2011), 492 , 151-188.
linkJin,
Lihua; Amaya-Mazo, Xylenia; Apel, Matthew E.; Sankisa, Sudha S.;
Johnson, Elissa; Zbyszynska, Monika A.; Han, Alexander; “Ca
2+ and Mg
2+ bind tetracycline with distinct stoichiometries and linked deprotonation,”
Biophysical Chemistry (2007), 128(2-3), 185-196.
linkJin,
Lihua; Xue, Wei-Feng; Fukayama, June Wong; Yetter, Jaclyn; Pickering,
Michael; Carey, Jannette; "3. Asymmetric allosteric activation of the
symmetric ArgR hexamer," Journal of Molecular Biology (2005), 346(1),
43-56.
link