The Department of Chemistry has a large inventory of instrumentation that is used to support the department's teaching and research initiatives. General instrumentation is centrally located in the core instrumentation labs on the third floor of McGowan-South while more specialized instrumentation can be found in individual faculty research labs. The department strongly believes that its students should be exposed to modern instrumentation. As such, students involved in research have direct access to research-grade instrumentation of the caliber they will likely see in their post-DePaul careers.
Click on the following instrumentation classes to see more about the department's instrumentation capabilities.
Atomic absorption spectroscopy
| Varian AA240FS Atomic Absorption Spectrometer |
|
Technical Description:
Air / acetylene flame.
Measures multiple elements in a sample.
Location:
McGowan South 324
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| Varian SPECTR AA-200 Atomic Absorption Spectrometer |
|
Technical Description:
Air / acetylene flame.
Flame and Graphite Furnace combo AA system.
Double beam optics with D2 background correction.
Location:
McGowan South 324
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Fluorescence spectroscopy
| Cary Eclipse |
|
Technical Description:
Schwarzchild collection optics capture the light from the Xenon flash lamp.
Double choppers ensure that the sample and reference beam strike the detector at the same point, eliminating non-uniformity of the detector.
Phase locked wavelength drive prevents peak shifts and peak suppression at high scan speeds.
Variable slits provide optimum control over spectral resolution.
Thermostatted multicell Peltier holder allows the simultaneous measurement of up to four samples with completecontrol over temperature ramping for thermal denaturation and renaturation studies of DNA via Fluorescence Resonance Energy Transfer (FRET).
Location:
McGowan South 324
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| Thermo Fisher Nanodrop 3300 |
 |
Minimum Sample Size: 1 µl
Excitation Maxima: UV: 365 nm, Blue: 470 nm, White: 460-650 nm
Wavelength Range: 400-750 nm
Wavelength Accuracy: 1 nm
Spectral Resolution: 8 nm (FWHM at Hg 546 nm)
Fluorescence Precision: < 5% CV (10nM fluorescein)
Detection Limit: < 1 fmol fluorescein
Measurement Cycle Time: 2-10 sec
Mass Detection Limit: 1 picogram/microliter of dsDNA
Location:
McGowan South 310
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Infrared spectroscopy
| ABB FT-IR FTLA 2000 |
|
Spectral Range in the mid-IR region 6500-350 cm-1
Are insensitive to moisture and perform outstanding sensitivity in the 6500 to 500 cm-1 range.
Built in non-hygroscopic KBr BK7 optical parts.
Quarts halogen source.
Equipped with a second integrated optical path compatible with most spectroscopic accessories.
Location:
McGowan South 324
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| ABB FT-IR MB 3000 |
|
Spectral range 485 to 8,500 cm-1
Resolution better than 0.7 cm-1
Apodized resolution adjustable 1 to 64 cm-1, in increments of 2
Maximum signal-to-noise ratio: 50,000: 1
Location:
McGowan South 328
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| ABB FT-IR MB 160D |
|
Sensitivity: Peak signal-to-noise ratio >100000: 1 rms with extended signal averaging
Baseline stability: <0.1% deviation in 100% line short-term. No long-term drift.
Temperature coefficient: <1% / °C at 8000 cm-1
Purge stability:< 0.0001A water vapor variation with minimal time delay using the unique Arid-Zone sample compartment
Resolution: better than 1 cm-1
Location:
McGowan South 315
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| BomemDA3.02 High Resolution FT-IR |
|
Location: McGowan South 315
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Nuclear Magnetic Resonance Spectroscopy
| Bruker 300 MHz Spectrometer |
|
300 MHz narrow bore magnet
5mm BBO probe with ATM
z-Pulsed Field Gradients
2H gradient shimming
Running XWINNMR 3.6 and ICONNMR
Typical measurements include:
1D 1H and 1D 13C (regular, APT, DEPT,…)
2D 1H-1H (mainly COSY and TOCSY with others available upon request)
2D 1H-13C (HSQC, HMBC, HSQC-TOCSY)
Location:
McGowan South 329
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Nanalysis NMReady 60P
|
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Operating Frequency: 60 MHz (1.4 T)
Magnet: Permanent, no cryogens
Nuclei: 1H, 19F, 1H/19F dual mode
Lock: Deuterium with non-deuterium options
Sample type: Standard 5mm NMR tubes
Compatibility: JCAMP-DX, Mestrelab MNova, LabVIEW
Resolution: Line-width at 50% ≤ 1.2 Hz
Sensitivity (SNR): 40:1 (EtBz 1% single scan)
Location:
McGowan South 329
|
UV-Visible Spectroscopy
| Varian Cary 50 UV-Vis Spectrometer |
|
Long lifetime Xenon flash lamp.
Maximum scan rate is 24,000 nm/ min
Wavelength range of 190 – 1100 nm.
Data collection rate of 80 points/sec, ideal for kinetic assays.
Can measure samples up to 3 Abs.
Available Accessories:
Single Cell Peltier for precise temperature control.
Fiber Optic Coupler and Microplate Reader
Locations:
McGowan South 220 and 311
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| Beckman Coulter DU 730 UV-VIS Spectrometer |
|
Spectral bandwidth of 3.0 nm
Touch screen user interface
Wavelength range from 190 to 1100 nm
Focused beam design
Automatic lamp turn off
Location:
McGowan South 220
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| Thermo Fisher Nanodrop 1000 |
|
Micro-volume spectrometer
Sample retention technology allows for sample volumes as small as 0.5 µL.
Direct measurements in less than 5 sec.
Large concentration range (2 ng/µL -15,000 ng/µL)
Location:
McGowan South 310
|
| Thermo Fisher Nanodrop Lite |
|
Sample sample volumes as small as 0.5 µL.
Spectral Resolution: ≤8.0 nm
Available wavelengths: 260 and 280 nm only
Lower Limit of Detection: 4 ng/µl (dsDNA), 0.12 mg/ml (BSA)
Maximum Concentration: 1,500 ng/µl (dsDNA), 45 mg/ml (BSA)
Absorbance Range (10 mm equivalent): 0 - 30 Abs
Location:
McGowan South 220
|
Atomic absorption spectroscopy
| Varian AA240FS Atomic Absorption Spectrometer |
|
Technical Description:
Air / acetylene flame.
Measures multiple elements in a sample.
Location:
McGowan South 324 |
| Varian SPECTR AA-200 Atomic Absorption Spectrometer |
|
Technical Description:
Air / acetylene flame.
Flame and Graphite Furnace combo AA system.
Double beam optics with D2 background correction.
Location:
McGowan South 324 |
Fluorescence spectroscopy
| Cary Eclipse |
|
Technical Description:
Schwarzchild collection optics capture the light from the Xenon flash lamp.
Double choppers ensure that the sample and reference beam strike the detector at the same point, eliminating non-uniformity of the detector.
Phase locked wavelength drive prevents peak shifts and peak suppression at high scan speeds.
Variable slits provide optimum control over spectral resolution.
Thermostatted multicell Peltier holder allows the simultaneous measurement of up to four samples with completecontrol over temperature ramping for thermal denaturation and renaturation studies of DNA via Fluorescence Resonance Energy Transfer (FRET).
Location:
McGowan South 324 |
| Thermo Fisher Nanodrop 3300 |
|
Minimum Sample Size: 1 µl
Excitation Maxima: UV: 365 nm, Blue: 470 nm, White: 460-650 nm
Wavelength Range: 400-750 nm
Wavelength Accuracy: 1 nm
Spectral Resolution: 8 nm (FWHM at Hg 546 nm)
Fluorescence Precision: < 5% CV (10nM fluorescein)
Detection Limit: < 1 fmol fluorescein
Measurement Cycle Time: 2-10 sec
Mass Detection Limit: 1 picogram/microliter of dsDNA
Location:
McGowan South 310 |
Infrared spectroscopy
| ABB FT-IR FTLA 2000 |
|
Spectral Range in the mid-IR region 6500-350 cm-1
Are insensitive to moisture and perform outstanding sensitivity in the 6500 to 500 cm-1 range.
Built in non-hygroscopic KBr BK7 optical parts.
Quarts halogen source.
Equipped with a second integrated optical path compatible with most spectroscopic accessories.
Location:
McGowan South 324 |
| ABB FT-IR MB 3000 |
|
Spectral range 485 to 8,500 cm-1
Resolution better than 0.7 cm-1
Apodized resolution adjustable 1 to 64 cm-1, in increments of 2
Maximum signal-to-noise ratio: 50,000: 1
Location:
McGowan South 328 |
| ABB FT-IR MB 160D |
|
Sensitivity: Peak signal-to-noise ratio >100000: 1 rms with extended signal averaging
Baseline stability: <0.1% deviation in 100% line short-term. No long-term drift.
Temperature coefficient: <1% / °C at 8000 cm-1
Purge stability:< 0.0001A water vapor variation with minimal time delay using the unique Arid-Zone sample compartment
Resolution: better than 1 cm-1
Location:
McGowan South 315 |
| BomemDA3.02 High Resolution FT-IR |
|
Location: McGowan South 315 |
Nuclear Magnetic Resonance Spectroscopy
| Bruker 300 MHz Spectrometer |
|
300 MHz narrow bore magnet
5mm BBO probe with ATM
z-Pulsed Field Gradients
2H gradient shimming
Running XWINNMR 3.6 and ICONNMR
Typical measurements include:
1D 1H and 1D 13C (regular, APT, DEPT,…)
2D 1H-1H (mainly COSY and TOCSY with others available upon request)
2D 1H-13C (HSQC, HMBC, HSQC-TOCSY)
Location:
McGowan South 329 |
Nanalysis NMReady 60P
|
|
Operating Frequency: 60 MHz (1.4 T)
Magnet: Permanent, no cryogens
Nuclei: 1H, 19F, 1H/19F dual mode
Lock: Deuterium with non-deuterium options
Sample type: Standard 5mm NMR tubes
Compatibility: JCAMP-DX, Mestrelab MNova, LabVIEW
Resolution: Line-width at 50% ≤ 1.2 Hz
Sensitivity (SNR): 40:1 (EtBz 1% single scan)
Location:
McGowan South 329 |
UV-Visible Spectroscopy
| Varian Cary 50 UV-Vis Spectrometer |
|
Long lifetime Xenon flash lamp.
Maximum scan rate is 24,000 nm/ min
Wavelength range of 190 – 1100 nm.
Data collection rate of 80 points/sec, ideal for kinetic assays.
Can measure samples up to 3 Abs.
Available Accessories:
Single Cell Peltier for precise temperature control.
Fiber Optic Coupler and Microplate Reader
Locations:
McGowan South 220 and 311 |
| Beckman Coulter DU 730 UV-VIS Spectrometer |
|
Spectral bandwidth of 3.0 nm
Touch screen user interface
Wavelength range from 190 to 1100 nm
Focused beam design
Automatic lamp turn off
Location:
McGowan South 220 |
| Thermo Fisher Nanodrop 1000 |
|
Micro-volume spectrometer
Sample retention technology allows for sample volumes as small as 0.5 µL.
Direct measurements in less than 5 sec.
Large concentration range (2 ng/µL -15,000 ng/µL)
Location:
McGowan South 310 |
| Thermo Fisher Nanodrop Lite |
|
Sample sample volumes as small as 0.5 µL.
Spectral Resolution: ≤8.0 nm
Available wavelengths: 260 and 280 nm only
Lower Limit of Detection: 4 ng/µl (dsDNA), 0.12 mg/ml (BSA)
Maximum Concentration: 1,500 ng/µl (dsDNA), 45 mg/ml (BSA)
Absorbance Range (10 mm equivalent): 0 - 30 Abs
Location:
McGowan South 220 |
Cell dismembrator |
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Fisher 500
Location:
McGowan South 324 |
Freeze-drying |
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Labconco Freezone 1
Location:
McGowan South 324 |
Gloveboxes |
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MBraun
Location:
McGowan South 324 |
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MBraun
Location:
McGowan South 324 |
Incubators |
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New Brunswick Scientific Innova 44
Location:
McGowan South 324
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New Brunswick Scientific Innova E24
Location:
McGowan South 324 |
Mechanical testers |
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MTS Qtest FLS 210
Location:
McGowan South 324 |
Sterilization |
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Harvey Sterilemax ST75925
Location:
McGowan South 324 |
Sputtering |
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Spelco SC-7
Location:
McGowan South 324 |
Sonification |
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Fisher Scientific FS110
Location:
McGowan South 324 |
PCR |
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Eppendorf Mastercycler Pro S
Location:
McGowan South 324 |
Ovens |
| image |
Fisher Isotemp 675
Location:
McGowan South 324 |
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Labline Model 3510
Location:
McGowan South 324 |
Temperature controlled rooms |
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Cold room (4 °C)
Location:
McGowan South 318B |
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Warm room (37 °C)
Location:
McGowan South 318A |
Flash Chromatography
| Teledyne Isco CombiFlash Rf |
|
Location:
McGowan South 324
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Gas Chromatography
| Varian CP-3800 |
|
Location:
McGowan South 324
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| Agilent 7820A with 5977E Mass Spectrometer |
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Location:
McGowan South 324
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| Agilent 6890 with 5975 Mass Spectrometer |
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Location:
McGowan South 324
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High Performance Liquid Chromatography
| Waters Acquity UPLC |
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Location:
McGowan South 309B
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| Rainin Dynamax Preparative HPLC |
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Maximum flow rate: 25 mL/min
Detector: Single wavelenth UV-Vis
Location:
McGowan South 309B
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