Lihua Jin

My research focuses on the study of biomolecular interactions using Isothermal Titration Calorimetry (ITC).  ITC directly measures the heat associated with most biomolecular interactions to determine thermodynamic parameters including binding affinity, stoichiometry, and binding energetics to lead us to a better understanding of molecular interactions. Systems studied so far include the interactions of proteins, peptides, antibiotics, amino acids, drugs and drug candidates with small molecules and metal ions.  Such studies also contribute to better drug designs and testing. 

Selected Publications

Jin, L., Sakiyan, I., Gonzales, N. S., Lane, D., and Cherala, S. S. “Synthesis, Characterization and Cu²⁺ Binding Studies of L-Histidine Ester of 8-Hydroxyquinoline” Inorg. Chim. Acta, (2014) link

Strawn, Rebecca; Stockner, Thomas; Melichercik, Milan; Jin, Lihua; Xue, Wei-Feng; Carey, Jannette; Ettrich, Rudiger; "Synergy of molecular dynamics and isothermal titration calorimetry in studies of allostery;" Methods in Enzymology (2011), 492 , 151-188. link

Jin, Lihua; Amaya-Mazo, Xylenia; Apel, Matthew E.; Sankisa, Sudha S.; Johnson, Elissa; Zbyszynska, Monika A.; Han, Alexander; “Ca²⁺ and Mg²⁺ bind tetracycline with distinct stoichiometries and linked deprotonation,” Biophysical Chemistry (2007), 128(2-3), 185-196. link

Jin, Lihua; Xue, Wei-Feng; Fukayama, June Wong; Yetter, Jaclyn; Pickering, Michael; Carey, Jannette; "3. Asymmetric allosteric activation of the symmetric ArgR hexamer," Journal of Molecular Biology (2005), 346(1), 43-56. link