Instrumentation
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The Department of Chemistry has a large inventory of instrumentation that is used to support the department's teaching and research initiatives. General instrumentation is centrally located in the core instrumentation labs on the third floor of McGowan-South while more specialized instrumentation can be found in individual faculty research labs. The department strongly believes that its students should be exposed to modern instrumentation. As such, students involved in research have direct access to research-grade instrumentation of the caliber they will likely see in their post-DePaul careers.
Click on the following instrumentation classes to see more about the department's instrumentation capabilities.
Spectroscopy
Atomic absorption spectroscopy
Varian AA240FS Atomic Absorption Spectrometer Technical Description:
Air / acetylene flame.
Measures multiple elements in a sample.Location:
McGowan South 324Varian SPECTR AA-200 Atomic Absorption Spectrometer Technical Description:
Air / acetylene flame.
Flame and Graphite Furnace combo AA system.
Double beam optics with D2 background correction.Location:
McGowan South 324
Fluorescence spectroscopy
Cary Eclipse Technical Description:
Schwarzchild collection optics capture the light from the Xenon flash lamp.
Double choppers ensure that the sample and reference beam strike the detector at the same point, eliminating non-uniformity of the detector.
Phase locked wavelength drive prevents peak shifts and peak suppression at high scan speeds.
Variable slits provide optimum control over spectral resolution.
Thermostatted multicell Peltier holder allows the simultaneous measurement of up to four samples with completecontrol over temperature ramping for thermal denaturation and renaturation studies of DNA via Fluorescence Resonance Energy Transfer (FRET).Location:
McGowan South 324Thermo Fisher Nanodrop 3300 Minimum Sample Size: 1 µl
Excitation Maxima: UV: 365 nm, Blue: 470 nm, White: 460-650 nm
Wavelength Range: 400-750 nm
Wavelength Accuracy: 1 nm
Spectral Resolution: 8 nm (FWHM at Hg 546 nm)
Fluorescence Precision: < 5%="" cv="" (10nm="">
Detection Limit: < 1="" fmol="">
Measurement Cycle Time: 2-10 sec
Mass Detection Limit: 1 picogram/microliter of dsDNALocation:
McGowan South 310
Infrared spectroscopy
ABB FT-IR FTLA 2000 Spectral Range in the mid-IR region 6500-350 cm-1
Are insensitive to moisture and perform outstanding sensitivity in the 6500 to 500 cm-1 range.
Built in non-hygroscopic KBr BK7 optical parts.
Quarts halogen source.
Equipped with a second integrated optical path compatible with most spectroscopic accessories.Location:
McGowan South 324ABB FT-IR MB 3000 Spectral range 485 to 8,500 cm-1
Resolution better than 0.7 cm-1
Apodized resolution adjustable 1 to 64 cm-1, in increments of 2
Maximum signal-to-noise ratio: 50,000: 1Location:
McGowan South 328ABB FT-IR MB 160D Sensitivity: Peak signal-to-noise ratio >100000: 1 rms with extended signal averaging
Baseline stability: <0.1% deviation="" in="" 100%="" line="" short-term.="" no="" long-term="">
Temperature coefficient: <1% °c="" at="" 8000="">-1
Purge stability:< 0.0001a="" water="" vapor="" variation="" with="" minimal="" time="" delay="" using="" the="" unique="" arid-zone="" sample="">
Resolution: better than 1 cm-1Location:
McGowan South 315BomemDA3.02 High Resolution FT-IR Location: McGowan South 315
Nuclear Magnetic Resonance Spectroscopy
Bruker 300 MHz Spectrometer 300 MHz narrow bore magnet
5mm BBO probe with ATM
z-Pulsed Field Gradients
2H gradient shimming
Running XWINNMR 3.6 and ICONNMRTypical measurements include:
1D 1H and 1D 13C (regular, APT, DEPT,…)
2D 1H-1H (mainly COSY and TOCSY with others available upon request)
2D 1H-13C (HSQC, HMBC, HSQC-TOCSY)Location:
McGowan South 329Nanalysis NMReady 60P Operating Frequency: 60 MHz (1.4 T)
Magnet: Permanent, no cryogens
Nuclei: 1H, 19F, 1H/19F dual mode
Lock: Deuterium with non-deuterium options
Sample type: Standard 5mm NMR tubes
Compatibility: JCAMP-DX, Mestrelab MNova, LabVIEW
Resolution: Line-width at 50% ≤ 1.2 Hz
Sensitivity (SNR): 40:1 (EtBz 1% single scan)Location:
McGowan South 329
UV-Visible Spectroscopy
Varian Cary 50 UV-Vis Spectrometer Long lifetime Xenon flash lamp.
Maximum scan rate is 24,000 nm/ min
Wavelength range of 190 – 1100 nm.
Data collection rate of 80 points/sec, ideal for kinetic assays.
Can measure samples up to 3 Abs.Available Accessories:
Single Cell Peltier for precise temperature control.
Fiber Optic Coupler and Microplate ReaderLocations:
McGowan South 220 and 311Beckman Coulter DU 730 UV-VIS Spectrometer Spectral bandwidth of 3.0 nm
Touch screen user interface
Wavelength range from 190 to 1100 nm
Focused beam design
Automatic lamp turn offLocation:
McGowan South 220Thermo Fisher Nanodrop 1000 Micro-volume spectrometer
Sample retention technology allows for sample volumes as small as 0.5 µL.
Direct measurements in less than 5 sec.
Large concentration range (2 ng/µL -15,000 ng/µL)Location:
McGowan South 310Thermo Fisher Nanodrop Lite Sample sample volumes as small as 0.5 µL.
Spectral Resolution: ≤8.0 nm
Available wavelengths: 260 and 280 nm only
Lower Limit of Detection: 4 ng/µl (dsDNA), 0.12 mg/ml (BSA)
Maximum Concentration: 1,500 ng/µl (dsDNA), 45 mg/ml (BSA)
Absorbance Range (10 mm equivalent): 0 - 30 AbsLocation:
McGowan South 220
Spectroscopy
Atomic absorption spectroscopy
Varian AA240FS Atomic Absorption Spectrometer Technical Description:
Air / acetylene flame.
Measures multiple elements in a sample.Location:
McGowan South 324Varian SPECTR AA-200 Atomic Absorption Spectrometer Technical Description:
Air / acetylene flame.
Flame and Graphite Furnace combo AA system.
Double beam optics with D2 background correction.Location:
McGowan South 324
Fluorescence spectroscopy
Cary Eclipse Technical Description:
Schwarzchild collection optics capture the light from the Xenon flash lamp.
Double choppers ensure that the sample and reference beam strike the detector at the same point, eliminating non-uniformity of the detector.
Phase locked wavelength drive prevents peak shifts and peak suppression at high scan speeds.
Variable slits provide optimum control over spectral resolution.
Thermostatted multicell Peltier holder allows the simultaneous measurement of up to four samples with completecontrol over temperature ramping for thermal denaturation and renaturation studies of DNA via Fluorescence Resonance Energy Transfer (FRET).Location:
McGowan South 324Thermo Fisher Nanodrop 3300 Minimum Sample Size: 1 µl
Excitation Maxima: UV: 365 nm, Blue: 470 nm, White: 460-650 nm
Wavelength Range: 400-750 nm
Wavelength Accuracy: 1 nm
Spectral Resolution: 8 nm (FWHM at Hg 546 nm)
Fluorescence Precision: < 5%="" cv="" (10nm="">
Detection Limit: < 1="" fmol="">
Measurement Cycle Time: 2-10 sec
Mass Detection Limit: 1 picogram/microliter of dsDNALocation:
McGowan South 310
Infrared spectroscopy
ABB FT-IR FTLA 2000 Spectral Range in the mid-IR region 6500-350 cm-1
Are insensitive to moisture and perform outstanding sensitivity in the 6500 to 500 cm-1 range.
Built in non-hygroscopic KBr BK7 optical parts.
Quarts halogen source.
Equipped with a second integrated optical path compatible with most spectroscopic accessories.
Location:
McGowan South 324ABB FT-IR MB 3000 Spectral range 485 to 8,500 cm-1
Resolution better than 0.7 cm-1
Apodized resolution adjustable 1 to 64 cm-1, in increments of 2
Maximum signal-to-noise ratio: 50,000: 1Location:
McGowan South 328ABB FT-IR MB 160D Sensitivity: Peak signal-to-noise ratio >100000: 1 rms with extended signal averaging
Baseline stability: <0.1% deviation="" in="" 100%="" line="" short-term.="" no="" long-term="">
Temperature coefficient: <1% °c="" at="" 8000="">-1
Purge stability:< 0.0001a="" water="" vapor="" variation="" with="" minimal="" time="" delay="" using="" the="" unique="" arid-zone="" sample="">
Resolution: better than 1 cm-1Location:
McGowan South 315BomemDA3.02 High Resolution FT-IR Location: McGowan South 315
Nuclear Magnetic Resonance Spectroscopy
Bruker 300 MHz Spectrometer 300 MHz narrow bore magnet
5mm BBO probe with ATM
z-Pulsed Field Gradients
2H gradient shimming
Running XWINNMR 3.6 and ICONNMRTypical measurements include:
1D 1H and 1D 13C (regular, APT, DEPT,…)
2D 1H-1H (mainly COSY and TOCSY with others available upon request)
2D 1H-13C (HSQC, HMBC, HSQC-TOCSY)Location:
McGowan South 329Nanalysis NMReady 60P Operating Frequency: 60 MHz (1.4 T)
Magnet: Permanent, no cryogens
Nuclei: 1H, 19F, 1H/19F dual mode
Lock: Deuterium with non-deuterium options
Sample type: Standard 5mm NMR tubes
Compatibility: JCAMP-DX, Mestrelab MNova, LabVIEW
Resolution: Line-width at 50% ≤ 1.2 Hz
Sensitivity (SNR): 40:1 (EtBz 1% single scan)Location:
McGowan South 329
UV-Visible Spectroscopy
Varian Cary 50 UV-Vis Spectrometer Long lifetime Xenon flash lamp.
Maximum scan rate is 24,000 nm/ min
Wavelength range of 190 – 1100 nm.
Data collection rate of 80 points/sec, ideal for kinetic assays.
Can measure samples up to 3 Abs.Available Accessories:
Single Cell Peltier for precise temperature control.
Fiber Optic Coupler and Microplate ReaderLocations:
McGowan South 220 and 311Beckman Coulter DU 730 UV-VIS Spectrometer Spectral bandwidth of 3.0 nm
Touch screen user interface
Wavelength range from 190 to 1100 nm
Focused beam design
Automatic lamp turn offLocation:
McGowan South 220Thermo Fisher Nanodrop 1000 Micro-volume spectrometer
Sample retention technology allows for sample volumes as small as 0.5 µL.
Direct measurements in less than 5 sec.
Large concentration range (2 ng/µL -15,000 ng/µL)Location:
McGowan South 310
Thermo Fisher Nanodrop Lite Sample sample volumes as small as 0.5 µL.
Spectral Resolution: ≤8.0 nm
Available wavelengths: 260 and 280 nm only
Lower Limit of Detection: 4 ng/µl (dsDNA), 0.12 mg/ml (BSA)
Maximum Concentration: 1,500 ng/µl (dsDNA), 45 mg/ml (BSA)
Absorbance Range (10 mm equivalent): 0 - 30 AbsLocation:
McGowan South 220Other major equipment
Cell dismembratorimage Fisher 500
Location:
McGowan South 324Freeze-dryingimage Labconco Freezone 1
Location:
McGowan South 324Gloveboxesimage MBraun
Location:
McGowan South 324image MBraun
Location:
McGowan South 324Incubatorsimage New Brunswick Scientific Innova 44
Location:
McGowan South 324image New Brunswick Scientific Innova E24
Location:
McGowan South 324Mechanical testersimage MTS Qtest FLS 210
Location:
McGowan South 324Sterilizationimage Harvey Sterilemax ST75925
Location:
McGowan South 324Sputteringimage Spelco SC-7
Location:
McGowan South 324Sonificationimage Fisher Scientific FS110
Location:
McGowan South 324PCRimage Eppendorf Mastercycler Pro S
Location:
McGowan South 324Ovensimage Fisher Isotemp 675
Location:
McGowan South 324image Labline Model 3510
Location:
McGowan South 324Temperature controlled roomsimage Cold room (4 °C)
Location:
McGowan South 318Bimage Warm room (37 °C)
Location:
McGowan South 318AChromatography
Flash Chromatography
Teledyne Isco CombiFlash Rf Location:
McGowan South 324
Gas Chromatography
Agilent 7820A Varian CP-3800 Location:
McGowan South 324Agilent 7820A with 5977E Mass Spectrometer Location:
McGowan South 324Agilent 6890 with 5975 Mass Spectrometer Location:
McGowan South 324
High Performance Liquid Chromatography
Waters Acquity UPLC Location:
McGowan South 309BRainin Dynamax Preparative HPLC
Maximum flow rate: 25 mL/min
Detector: Single wavelenth UV-VisLocation:
McGowan South 309B
