College of Science and Health | DePaul University > Academics > Chemistry > Facilities > Instrumentation

Instrumentation

The Department of Chemistry has a large inventory of instrumentation that is used to support the department's teaching and research initiatives.  General instrumentation is centrally located in the core instrumentation labs on the third floor of McGowan-South while more specialized instrumentation can be found in individual faculty research labs.  The department strongly believes that its students should be exposed to modern instrumentation.  As such, students involved in research have direct access to research-grade instrumentation of the caliber they will likely see in their post-DePaul careers.

Click on the following instrumentation classes to see more about the department's instrumentation capabilities.

Chromatography

Atomic absorption spectroscopy

Varian AA240FS Atomic Absorption Spectrometer
Varian AA240FS

Technical Description:
Air / acetylene flame.
Measures multiple elements in a sample.

Location:
McGowan South 324

 

Varian SPECTR AA-200 Atomic Absorption Spectrometer
Varian AA200

Technical Description:
Air / acetylene flame.
Flame and Graphite Furnace combo AA system.
Double beam optics with D2 background correction.

Location:
McGowan South 324


 

Fluorescence spectroscopy 

Cary Eclipse
Varian Cary Eclipse

Technical Description:
Schwarzchild collection optics capture the light from the Xenon flash lamp.
Double choppers ensure that the sample and reference beam strike the detector at the same point, eliminating non-uniformity of the detector.
Phase locked wavelength drive prevents peak shifts and peak suppression at high scan speeds.
Variable slits provide optimum control over spectral resolution.
Thermostatted multicell Peltier holder allows the simultaneous measurement of up to four samples with completecontrol over temperature ramping for thermal denaturation and renaturation studies of DNA via Fluorescence Resonance Energy Transfer (FRET).

Location:
McGowan South 324

 

Thermo Fisher Nanodrop 3300

Minimum Sample Size: 1 µl
Excitation Maxima: UV: 365 nm, Blue: 470 nm, White: 460-650 nm
Wavelength Range: 400-750 nm
Wavelength Accuracy: 1 nm
Spectral Resolution: 8 nm (FWHM at Hg 546 nm)
Fluorescence Precision: < 5% CV (10nM fluorescein)
Detection Limit: < 1 fmol fluorescein
Measurement Cycle Time: 2-10 sec
Mass Detection Limit: 1 picogram/microliter of dsDNA

Location:
McGowan South 310


 

Infrared spectroscopy

ABB FT-IR FTLA 2000

ABB FTLA2000

Spectral Range in the mid-IR region 6500-350 cm-1
Are insensitive to moisture and perform outstanding sensitivity in the 6500 to 500 cm-1 range.
Built in non-hygroscopic KBr BK7 optical parts.
Quarts halogen source.
Equipped with a second integrated optical path compatible with most spectroscopic accessories.

Location:
McGowan South 324

 

ABB FT-IR MB 3000
ABB MB 3000

Spectral range 485 to 8,500 cm-1
Resolution better than 0.7 cm-1
Apodized resolution adjustable 1 to 64 cm-1, in increments of 2
Maximum signal-to-noise ratio: 50,000: 1

Location:
McGowan South 328

 

ABB FT-IR MB 160D
ABB FT-IR MB 160D

Sensitivity: Peak signal-to-noise ratio >100000: 1 rms with extended signal averaging
Baseline stability: <0.1% deviation in 100% line short-term. No long-term drift.
Temperature coefficient: <1% / °C at 8000 cm-1
Purge stability:< 0.0001A water vapor variation with minimal time delay using the unique Arid-Zone sample compartment
Resolution: better than 1 cm-1

Location:
McGowan South 315

 

BomemDA3.02 High Resolution FT-IR
BomemDA3.02

Location: McGowan South 315

 


Nuclear Magnetic Resonance Spectroscopy

Bruker 300 MHz Spectrometer
Bruker NMR

300 MHz narrow bore magnet
5mm BBO probe with ATM
z-Pulsed Field Gradients
2H gradient shimming
Running XWINNMR 3.6 and ICONNMR

Typical measurements include:
1D 1H and 1D 13C (regular, APT, DEPT,…)
2D 1H-1H (mainly COSY and TOCSY with others available upon request)
2D 1H-13C (HSQC, HMBC, HSQC-TOCSY)

Location:
McGowan South 329

 

Nanalysis NMReady 60P


Operating Frequency: 60 MHz (1.4 T)
Magnet: Permanent, no cryogens
Nuclei: 1H, 19F, 1H/19F dual mode
Lock: Deuterium with non-deuterium options
Sample type: Standard 5mm NMR tubes
Compatibility: JCAMP-DX, Mestrelab MNova, LabVIEW
Resolution: Line-width at 50% ≤ 1.2 Hz
Sensitivity (SNR): 40:1 (EtBz 1% single scan)

Location:
McGowan South 329


 

UV-Visible Spectroscopy

Varian Cary 50 UV-Vis Spectrometer
Varian Cary 50

Long lifetime Xenon flash lamp.
Maximum scan rate is 24,000 nm/ min
Wavelength range of 190 – 1100 nm.
Data collection rate of 80 points/sec, ideal for kinetic assays.
Can measure samples up to 3 Abs.

Available Accessories:
Single Cell Peltier for precise temperature control.
Fiber Optic Coupler and Microplate Reader

Locations:
McGowan South 220 and 311

 

Beckman Coulter DU 730 UV-VIS Spectrometer
Beckman DU730

Spectral bandwidth of 3.0 nm
Touch screen user interface
Wavelength range from 190 to 1100 nm
Focused beam design
Automatic lamp turn off

Location:
McGowan South 220

 

Thermo Fisher Nanodrop 1000
NanoDrop 1000

Micro-volume spectrometer
Sample retention technology allows for sample volumes as small as 0.5 µL.
Direct measurements in less than 5 sec.
Large concentration range (2 ng/µL -15,000 ng/µL)

Location:
McGowan South 310

 

Thermo Fisher Nanodrop Lite
NanoDrop Lite

Sample sample volumes as small as 0.5 µL.
Spectral Resolution: ≤8.0 nm
Available wavelengths: 260 and 280 nm only
Lower Limit of Detection: 4 ng/µl (dsDNA), 0.12 mg/ml (BSA)
Maximum Concentration: 1,500 ng/µl (dsDNA), 45 mg/ml (BSA)
Absorbance Range (10 mm equivalent): 0 - 30 Abs

Location:
McGowan South 220



Spectroscopy

Atomic absorption spectroscopy

Varian AA240FS Atomic Absorption Spectrometer
Varian AA240FS

Technical Description:
Air / acetylene flame.
Measures multiple elements in a sample.

Location:
McGowan South 324

 

Varian SPECTR AA-200 Atomic Absorption Spectrometer
Varian AA200

Technical Description:
Air / acetylene flame.
Flame and Graphite Furnace combo AA system.
Double beam optics with D2 background correction.

Location:
McGowan South 324


 

Fluorescence spectroscopy 

Cary Eclipse
Varian Cary Eclipse

Technical Description:
Schwarzchild collection optics capture the light from the Xenon flash lamp.
Double choppers ensure that the sample and reference beam strike the detector at the same point, eliminating non-uniformity of the detector.
Phase locked wavelength drive prevents peak shifts and peak suppression at high scan speeds.
Variable slits provide optimum control over spectral resolution.
Thermostatted multicell Peltier holder allows the simultaneous measurement of up to four samples with completecontrol over temperature ramping for thermal denaturation and renaturation studies of DNA via Fluorescence Resonance Energy Transfer (FRET).

Location:
McGowan South 324

 

Thermo Fisher Nanodrop 3300

Minimum Sample Size: 1 µl
Excitation Maxima: UV: 365 nm, Blue: 470 nm, White: 460-650 nm
Wavelength Range: 400-750 nm
Wavelength Accuracy: 1 nm
Spectral Resolution: 8 nm (FWHM at Hg 546 nm)
Fluorescence Precision: < 5% CV (10nM fluorescein)
Detection Limit: < 1 fmol fluorescein
Measurement Cycle Time: 2-10 sec
Mass Detection Limit: 1 picogram/microliter of dsDNA

Location:
McGowan South 310


 

Infrared spectroscopy

ABB FT-IR FTLA 2000

ABB FTLA2000

Spectral Range in the mid-IR region 6500-350 cm-1
Are insensitive to moisture and perform outstanding sensitivity in the 6500 to 500 cm-1 range.
Built in non-hygroscopic KBr BK7 optical parts.
Quarts halogen source.
Equipped with a second integrated optical path compatible with most spectroscopic accessories.

Location:
McGowan South 324

 

ABB FT-IR MB 3000
ABB MB 3000

Spectral range 485 to 8,500 cm-1
Resolution better than 0.7 cm-1
Apodized resolution adjustable 1 to 64 cm-1, in increments of 2
Maximum signal-to-noise ratio: 50,000: 1

Location:
McGowan South 328

 

ABB FT-IR MB 160D
ABB FT-IR MB 160D

Sensitivity: Peak signal-to-noise ratio >100000: 1 rms with extended signal averaging
Baseline stability: <0.1% deviation in 100% line short-term. No long-term drift.
Temperature coefficient: <1% / °C at 8000 cm-1
Purge stability:< 0.0001A water vapor variation with minimal time delay using the unique Arid-Zone sample compartment
Resolution: better than 1 cm-1

Location:
McGowan South 315

 

BomemDA3.02 High Resolution FT-IR
BomemDA3.02

Location: McGowan South 315

 


Nuclear Magnetic Resonance Spectroscopy

Bruker 300 MHz Spectrometer
Bruker NMR

300 MHz narrow bore magnet
5mm BBO probe with ATM
z-Pulsed Field Gradients
2H gradient shimming
Running XWINNMR 3.6 and ICONNMR

Typical measurements include:
1D 1H and 1D 13C (regular, APT, DEPT,…)
2D 1H-1H (mainly COSY and TOCSY with others available upon request)
2D 1H-13C (HSQC, HMBC, HSQC-TOCSY)

Location:
McGowan South 329

 

Nanalysis NMReady 60P


Operating Frequency: 60 MHz (1.4 T)
Magnet: Permanent, no cryogens
Nuclei: 1H, 19F, 1H/19F dual mode
Lock: Deuterium with non-deuterium options
Sample type: Standard 5mm NMR tubes
Compatibility: JCAMP-DX, Mestrelab MNova, LabVIEW
Resolution: Line-width at 50% ≤ 1.2 Hz
Sensitivity (SNR): 40:1 (EtBz 1% single scan)

Location:
McGowan South 329


 

UV-Visible Spectroscopy

Varian Cary 50 UV-Vis Spectrometer
Varian Cary 50

Long lifetime Xenon flash lamp.
Maximum scan rate is 24,000 nm/ min
Wavelength range of 190 – 1100 nm.
Data collection rate of 80 points/sec, ideal for kinetic assays.
Can measure samples up to 3 Abs.

Available Accessories:
Single Cell Peltier for precise temperature control.
Fiber Optic Coupler and Microplate Reader

Locations:
McGowan South 220 and 311

 

Beckman Coulter DU 730 UV-VIS Spectrometer
Beckman DU730

Spectral bandwidth of 3.0 nm
Touch screen user interface
Wavelength range from 190 to 1100 nm
Focused beam design
Automatic lamp turn off

Location:
McGowan South 220

 

Thermo Fisher Nanodrop 1000
NanoDrop 1000

Micro-volume spectrometer
Sample retention technology allows for sample volumes as small as 0.5 µL.
Direct measurements in less than 5 sec.
Large concentration range (2 ng/µL -15,000 ng/µL)

Location:
McGowan South 310

 

Thermo Fisher Nanodrop Lite
NanoDrop Lite

Sample sample volumes as small as 0.5 µL.
Spectral Resolution: ≤8.0 nm
Available wavelengths: 260 and 280 nm only
Lower Limit of Detection: 4 ng/µl (dsDNA), 0.12 mg/ml (BSA)
Maximum Concentration: 1,500 ng/µl (dsDNA), 45 mg/ml (BSA)
Absorbance Range (10 mm equivalent): 0 - 30 Abs

Location:
McGowan South 220

Other major equipment

Cell dismembrator
image

Fisher 500

Location:
McGowan South 324

 

Freeze-drying
image

Labconco Freezone 1

Location:
McGowan South 324

 

Gloveboxes
image

MBraun

Location:
McGowan South 324

image

MBraun

Location:
McGowan South 324

 

Incubators
image

New Brunswick Scientific Innova 44

Location:
McGowan South 324

 

image

New Brunswick Scientific Innova E24

Location:
McGowan South 324

 

Mechanical testers
image

MTS Qtest FLS 210

Location:
McGowan South 324

 

Sterilization
image

Harvey Sterilemax ST75925

Location:
McGowan South 324

 

Sputtering
image

Spelco SC-7

Location:
McGowan South 324

 

Sonification
image

Fisher Scientific FS110

Location:
McGowan South 324

 

PCR
image

Eppendorf Mastercycler Pro S

Location:
McGowan South 324

 

Ovens
image

Fisher Isotemp 675

Location:
McGowan South 324

image

Labline Model 3510

Location:
McGowan South 324

 

Temperature controlled rooms
image

Cold room (4 °C)

Location:
McGowan South 318B

image

Warm room (37 °C)

Location:
McGowan South 318A

 

Chromatography

Flash Chromatography

Teledyne Isco CombiFlash Rf
CombiFlash Rf

 

Location:
McGowan South 324


 

Gas Chromatography

Agilent 7820A
Aglilent 7820A

 

Location:
McGowan South 324

 

Varian CP-3800
Varian CP-3800

 

Location:
McGowan South 324

 

Agilent 7820A with 5977E Mass Spectrometer

Aglilent 7820A-5977E

 

Location:
McGowan South 324

 

Agilent 6890 with 5975 Mass Spectrometer

 

Location:
McGowan South 324


 

High Performance Liquid Chromatography  

Waters Acquity UPLC
Water Acquity


 

Location:
McGowan South 309B

 

Rainin Dynamax Preparative HPLC

Maximum flow rate: 25 mL/min
Detector: Single wavelenth UV-Vis

Location:
McGowan South 309B